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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Cell Rep. 2014 Oct 2;9(1):180–192. doi: 10.1016/j.celrep.2014.08.074

Figure 4. Accumulated DNA activates Sting-dependent inflammation.

Figure 4

(A) Expression of Cxcl10 mRNA in untreated or Ara-C treated wild-type and Dnase2a−/− MLFs.

(B) Cxcl10 expression of Dnase2a−/− MLFs rescued with Dnase2a ORF using a Tet-on vector with doxycycline induction (3 μg/ml, 24 h); eGFP ORF was used as negative control.

(C) Immunostaining of anti-dsDNA for KO and rescued cells (as in B); scale bar, 20 μm; right panel, quantitation of fluorescent signals based on 5 fields of 20X.

(D) Cxcl10 mRNA levels in Dnase2a+/+ and Dnase2a−/− MLFs transfected with siRNAs targeting Sting or Tbk1, untreated or treated with 10 μM Ara-C for 24 h.

(E) Cxcl10 expression of KO or rescued MLFs after doxycycline induction, untreated or treated with Ara-C and leptomycin (20 nM, 24 h) as indicated. Data are representative of two (B, C, D) or three (A, E) independent experiments.

See also Figure S4.