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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Glia. 2015 Jul 17;63(12):2285–2297. doi: 10.1002/glia.22893

Figure 6. Glutamate uptake in ventral spinal cord astrocytes is reduced in hGFAPTG mice compared to WT littermates.

Figure 6

A. Superimposed traces of glutamate transporter currents recorded from ventral horn astrocytes from WT (black, n = 15) and hGFAPTG (cyan, n = 9) astrocytes are shown. Cells were voltage clamped at −80 mV and responses were evoked by a 200 μM, 400 msec puff of glutamate. Bathing and puffing solutions contained 20 μM AP5, 20 μM bicuculline, 20 μM CNQX, 500 nM TTX and 100 μM CdCl2. Each trace shown is an average of three individual traces for that astrocyte. B. Averaged data across all WT (black) and hGFAPTG (cyan) responses are shown. C. Summary scatter plot of the glutamate evoked responses indicates a decrease in the hGFAPTG mice (−8.1 ± 4.1 pA, [n = 9]) relative to WT littermate controls (−53.2 ± 4.1 pA, [n = 15], Two tailed t-test, p = 0.0416).