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| Method | Hanging-drop vapour diffusion |
| Plate type | 24-well plates, Hampton Research |
| Temperature (K) | 293 |
| Protein concentration (mgml1) | 89 |
| Composition of protein buffer solution | 20mM sodium citrate pH 5.0, 150mM NaCl |
| Composition of reservoir solution | 1.9M ammonium phosphate, 16%(v/v) glycerol, 0.08M TrisHCl pH 8.0 |
| Volume and ratio of drop | 2l, 1:1 |
| Volume of reservoir (l) | 400 |
