Table 1. Macromolecule-production information.
Source organism | Homo sapiens |
DNA source | GenBank L19684.1 |
Forward primer | 5-GTTTGGATCCAGCCTCAAGATAGCCCCTG-3 |
Reverse primer | 5-CAAAAAAGCTTATGGTTTCGTGGGGTCGAC-3 |
Cloning vector | pSumo3 |
Expression vector | pSumo3 |
Expression host | E. coli BL21(DE3) |
Complete amino-acid sequence of the construct produced | SLKIAPANADFAFRFYYLIASETPGKNIFFSPLSISAAYAMLSLGACSHSRSQILEGLGFNLTELSESDVHRGFQHLLHTLNLPGHGLETRVGSALFLSHNLKFLAKFLNDTMAVYEAKLFHTNFYDTVGTIQLINDHVKKETRGKIVDLVSELKKDVLMVLVNYIYFKALWEKPFISSRTTPKDFYVDENTTVRVPMMLQDQEHHWYLHDRYLPCSVLRMDYKGDATVFFILPNQGKMREIEEVLTPEMLMRWNNLLRKRNFYKKLELHLPKFSISGSYVLDQILPRLGFTDLFSKWADLSGITKQQKLEASKSFHKATLDVDEAGTEAAAATSFAIKFFSAQTNRHILRFNRPFLVVIFSTSTQSVLFLGKVVDPTK |