Skip to main content
. 2015 Aug 25;71(Pt 9):1135–1138. doi: 10.1107/S2053230X15012893

Table 1. Macromolecule-production information.

The restriction-enzyme cleavage sites are underlined.

Source organism Homo sapiens
DNA source GenBank L19684.1
Forward primer 5-GTTTGGATCCAGCCTCAAGATAGCCCCTG-3
Reverse primer 5-CAAAAAAGCTTATGGTTTCGTGGGGTCGAC-3
Cloning vector pSumo3
Expression vector pSumo3
Expression host E. coli BL21(DE3)
Complete amino-acid sequence of the construct produced SLKIAPANADFAFRFYYLIASETPGKNIFFSPLSISAAYAMLSLGACSHSRSQILEGLGFNLTELSESDVHRGFQHLLHTLNLPGHGLETRVGSALFLSHNLKFLAKFLNDTMAVYEAKLFHTNFYDTVGTIQLINDHVKKETRGKIVDLVSELKKDVLMVLVNYIYFKALWEKPFISSRTTPKDFYVDENTTVRVPMMLQDQEHHWYLHDRYLPCSVLRMDYKGDATVFFILPNQGKMREIEEVLTPEMLMRWNNLLRKRNFYKKLELHLPKFSISGSYVLDQILPRLGFTDLFSKWADLSGITKQQKLEASKSFHKATLDVDEAGTEAAAATSFAIKFFSAQTNRHILRFNRPFLVVIFSTSTQSVLFLGKVVDPTK