Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
| Method | Sitting-drop vapour diffusion |
| Plate type | MRC2 plates, 96-well |
| Temperature (K) | 295 |
| Protein concentration (mgml1) | 10 |
| Buffer composition of protein solution | 10mM MES pH 6.2, 0.15M NaCl |
| Composition of reservoir solution | 35% tert-butanol |
| Volume and ratio of drop | 0.4l; 0.2:0.2 |
| Volume of reservoir (l) | 80 |
