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| Method | Hanging-drop vapor diffusion |
| Plate type | Hampton Research VDX 24-well plate |
| Temperature (K) | 293 |
| Protein concentration (mgml1) | 15.0 |
| Buffer composition of protein solution | 10mM Tris pH 7.5, 150mM NaCl |
| Composition of reservoir solution | 1.0M magnesium sulfate, 100mM Tris pH 7.5, 5% glycerol |
| Volume and ratio of drop | 2 + 2l |
| Volume of reservoir (ml) | 1 |
