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. 2015 Sep 1;3:128. doi: 10.3389/fbioe.2015.00128

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Copyright © 2015 Bastin, Yang and Heximer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RGS4 Shows preferential co-localization with Gαi3RC. HEK293 cells were co-transfected with RGS4-YFP (yellow), and either Gαi3 (WT)- or Gαi3 (RC)-CFP (blue) to assess the extent of co-localization using spinning disk confocal microscopy. The merged view is a composite two-channel view of cells expressing the two indicated constructs. Data are representative of at least 100 dual-transfected cells. Arrows indicate co-localization on intracellular endosomal structures between RGS4 and Gαi3. Scale bars represent 1 μm.