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Co-localization of Gαi3 with AGS3 in mammalian cells. HEK 293 cells were co-transfected AGS3-GFP (green) and Gαi3WT-CFP (blue) to assess the extent of co-localization using spinning disk confocal microscopy. The merged view is a composite two-channel view of cells expressing the two indicated constructs. Data are representative of at least 100 dual-transfected cells. Arrows indicate co-localization between AGS3 and Gαi3 on intracellular endosomal structures. Scale bars represent 1 μm.
