Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Sep 1;3:128. doi: 10.3389/fbioe.2015.00128

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015 Bastin, Yang and Heximer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Co-localization of Gαi3 with AGS3 and RGS4 is dependent on its activation state. HEK 293 cells were co-transfected with RGS4-RFP (red), AGS3-GFP (green), and either Gαi3WT-CFP (A) or Gαi3RC-CFP (B) (blue) to assess the extent of co-localization using spinning disk confocal microscopy. The merged view is a composite three-channel view of cells expressing all three constructs. Shown below are the indicated single channel views or double-channel combinations. Data are representative of at least 100 triple-transfected cells. Arrows indicate co-localization between RGS4 and Gαi3, whereas arrowheads indicate co-localization between AGS3 and Gαi3. Scale bars represent 1 μm.