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. 2015 Sep 1;6:445. doi: 10.3389/fimmu.2015.00445

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Copyright © 2015 Gerard and Gerard.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Final steps in cloning and identifying the human C5a receptor. (A) Tertiary screen of clone NPIIY-18 using ³²P-labeled oligonucleotide probe. (B) NPIIY-18 was transfected into COS7 cells and tested for binding of ¹²⁵I-C5a under competitive binding conditions. (C) The resulting binding isotherm and Scatchard analysis proving the clone’s identity.