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. Author manuscript; available in PMC: 2016 Aug 27.
Published in final edited form as: Cell. 2015 Aug 27;162(5):974–986. doi: 10.1016/j.cell.2015.07.011

Figure 4. Aza activates dsRNA sensors to induce interferon signaling.

Figure 4

A) Effects on IFNβ1 transcripts, at 24 hours, in HT29 recipient cells transfected with nucleic acid fractions, treated with RNaseIII, from A2780 as in Figure 3d,e. IFNβ1 transcripts were measured at 24 hours. * = p≤0.05 for fold change over untreated; ^ = p≤0.05 for Mock or Aza + versus - RNAseIII. B) Western blots for MDA5, RIG-I, and TLR3 in A2780 cells at four (Day 7) and seven (Day 10) days after Mock vs 500 nM Aza (Aza) for 3 days. C) Knockdown upon lentiviral infection, with puromycin selection, of A2780 and TykNu with shGFP, shTLR3, and shSTING hairpins. Immunoblotting with anti-TLR3, anti-STING and anti-β-Actin. Densitometry fold change, normalized to mock or shGFP, shown at the bottom of the gels. D) q-RT-PCR for ISGs from B and C. * = p≤0.05 Aza over Mock; ^ = p≤0.05 shGFP versus each shRNA sensor with mean fold change +/− S.E.M. of three biological replicates. See also Figure S4.