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. 2015 Aug 24;8:2227–2235. doi: 10.2147/OTT.S87976

Figure 3.

Figure 3

FOXA1 is identified as a functional target of miR-212 in HCC.

Notes: (A) qRT-PCR and (B) Western blot analysis of FOXA1 expression in HepG2 cells with miR-212 or control vectors transfection. n=3 independent experiments, *P<0.05. (C) miR-212 and its putative binding sequence in the 3′-UTR of FOXA1. The mutant miR-212 binding site was generated in the complementary site for the seed region of miR-212 (wt, wild type; mt, mutant type). (D) miR-212 significantly suppressed the luciferase activity that carried wt but not mt 3′-UTR of FOXA1. Anti-miR-212 led to a noticeable increase in luciferase activity of wt 3′-UTR of FOXA1. n=3 repeats with similar results, *P<0.05.

Abbreviations: FOXA1, forkhead box protein A1; HCC, hepatocellular carcinoma; miR-212, microRNA 212; qRT-PCR, quantitative reverse transcription-polymerase chain reaction; UTR, untranslated region; NC, negative control; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.