Figure 3. The mutational context is similar in both early and late SNVs.

a) Heat map showing the log-transformed values representing the fraction of each mutation type at each trinucleotide mutation context corrected for the frequency of each trinucleotide in the reference genome, as described in Nik-Zainal et al²⁷. The mutational contexts were calculated separately for the three subsets of SNVs per patient, i.e. 1. Barrett’s unique and not in EAC LOH, 2. common to Barrett’s esophagus and EAC, and 3. EAC unique and not in Barrett’s esophagus LOH. b) Mutational context plotted as a dot plot showing the enrichment of the trinucleotide mutational context information for every possible option for all 23 paired Barrett’s esophagus and EAC samples.