FIG. 6.

Histochemical staining of stacked PLA scaffolds containing 0% or 20% TCP seeded with human adipose-derived stem cells (hASC) for 28 days. (A) Hematoxylin and eosin staining showing cells growing in all layers. Dark lines between stacks show regions of highest cell density where hASC were seeded on nanofibrous PLA or PLA/TCP scaffolds before stacking within collagen gel. (B) Alizarin red staining showing calcium accretion (red) in the 20% TCP-loaded nanofibers and none in 0% TCP-loaded nanofibers. (C) Alcian blue staining showing increased hASC chondrogenesis in top three layers (0% TCP). (D) Immunofluorescence for collagen I shows increased expression in the bottom 20% TCP layers when compared to the superficial 0% layers. Color images available online at www.liebertpub.com/tea