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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Plast Reconstr Surg. 2015 Sep;136(3):328e–337e. doi: 10.1097/PRS.0000000000001538

Figure 2.

Figure 2

17-AAG down-regulates mRNA expression of type I collagen in KFs. (above) qRT–PCR analysis indicated that type I collagen mRNA levels in HDFs treated with 17-AAG treatment (10 μM) were not changed. However, type I collagen mRNA levels in TGF-β1 (10 ng/ml) treated HDFs were significantly decreased with 17-AAG treatment (10 μM) (*p<0.05) versus 17-AAG untreated HDFs. (center) Real time RT–PCR analysis indicated that type I collagen mRNA levels in KFs treated with 17-AAG treatment (10 μM) was significantly decreased (**p<0.01) compared with non-treated KFs. (below) Also, the type I/III collagen mRNA ratio was significantly decreased by 77% (**p<0.01) in KFs treated with 17-AAG treatment (10 μM). Each experiment was performed at least four times, Standard error bars are shown.