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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Exp Cell Res. 2015 May 7;335(1):115–122. doi: 10.1016/j.yexcr.2015.04.019

Figure 2. PKA and CDK5 phosphorylate either PDPN S167 or S171.

Figure 2

Peptide containing the entire intracellular region (VVMKKISGRFSP) of PDPN was incubated with PKA, CDK5, or both PKA and CDK5 along with [γ-32]ATP for 10 minutes. Representative MS/MS spectrum of PKA and CDK5 treated samples show doubly-charged ion (m/z 714.87) corresponding to the phosphorylated PDPN peptide sequence with phosphorylation at S167 or S171 as indicated. Observed y-ion and b-ion series confirming the peptide sequence and phosphorylation sites are also shown.