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. 2015 Sep 2;21:1036–1050.

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Copyright © 2015 Molecular Vision.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed.

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Gal-1 expression in the anterior eye segment and eye supernatants. A: Galectin-1 (Gal-1) immunoreactivity in the epithelial cells of the cornea, B: ciliary body, and C: iris in the control group. D-F: Lipopolysaccharide (LPS) group with intense immunostaining in the epithelium. G-I: LPS + recombinant Gal-1 (rGal-1) group showing diminished Gal-1 expression in epithelial cells. J: No immunostaining was detected in the negative control section (without primary antibody). Counterstain: hematoxylin. Bars: 20 µm. K: Densitometric analysis of Gal-1 expression in epithelial cells. L: Representative western blot. Equal loading was confirmed using anti–β-actin. Immunoreactive protein bands were semiquantified via densitometry and expressed in arbitrary units (a.u.) relative to β-actin. Values (a.u.) are presented as the mean ± SEM of sections analyzed (n = 5). *p<0.05, **p<0.01, ***p<0.001 versus control; ###p<0.001 versus LPS.