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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: J Acquir Immune Defic Syndr. 2015 Sep 1;70(1):1–8. doi: 10.1097/QAI.0000000000000700

Figure 2. Functionality of CE specific CD8 T-cell responses restricted by protective alleles as measured in an ex-vivo ICS assay.

Figure 2

PBMC from a chronically HIV infected donor (HLA-A: 0201/0205, HLA-B:0702/5703, HLA-C:0701/0702) were stimulated with 10uM peptide in presence of co-stimulatory antibodies and intracellular transport inhibitors for 12 hours at 37°C. Representative data showing IFN-γ and perforin production from CE specific CD8 T-cells in response to B*5801 restricted Env RF4 (FIY9= FYFSSPSIY) and B*5801 restricted Gag RF5 (LF9= LPLWEGQIF) peptides. Response to SEB is shown as a positive control. Using Fischer’s exact test, p-value for FIY9 response relative to media was 1.1 × 10−6 for IFN-γ, and 4.0 × 10−6 for perforin. For LF9 response, the p-values for media compared to IFN-γ and perforin were 2.7 × 10−5 and 4.4 × 10−5 respectively. This patient was on ART and the plasma viral load and absolute CD4 counts were 49 and 611 respectively.