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. 2015 Aug 31;10(8):e0136728. doi: 10.1371/journal.pone.0136728

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© 2015 Ahn et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — (A) HBV 1.2mer DNA of all the mutants was transfected into Huh7 cells, which were treated with ETV for 3 days. The intracellular HBV DNA level was determined by Southern blot analysis. A representative result was displayed. (B) The relative replication levels of each HBV mutant (no drug vs ETV treatment) was calculated based on the results displayed in Figs 2C and 4A. (C) The relative replication ability of the HBV mutants treated with ETV were determined by Southern blot analysis, and quantified by Phosphorimager (*, P < 0.05; **, P < 0.01; ***, P < 0.001). The relative replication level of each HBV construct was displayed as the mean value of at least three independent experiments.