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. 2015 Sep 1;23(5):428–433. doi: 10.4062/biomolther.2015.102

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Copyright © 2015, The Korean Society of Applied Pharmacology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 6. — Inhibitory effect of acetylshikonin on the anchorage-independent colony formation of PANC-1 human pancreatic cancer cells. Anchorage-independent colony formation assay of PANC-1 cells was conducted according to the protocol in the Materials and Methods. Representative figures were photographed (Upper Panel) and the average colony number (Lower Panel) was calculated from three independent experiments. Colony sizes were calculated using the Image J software program. The asterisk(s) indicate a significant statistical significance (^**p<0.01).