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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: J Immunol. 2009 Nov 16;183(11):7150–7160. doi: 10.4049/jimmunol.0901481

Table I.

Flow cytometric comparison of neonatal and adult responses to TLR stimulation.a

Monocytes - MC
Cytokine Parameter measured PAM LPS 3M-002 3M-003
Adult Neonate q-value Adult Neonate q-value Adult Neonate q-value Adult Neonate q-value
IL-12/23p40 % positive 10.1 ± 1.3 12.6 ± 0.9 n.s. 1.5 ± 0.1 2.2 ± 1.2 n.s. 6.1 ± 0.6 9.7 ± 1.1 n.s. 6.6 ± 0.8 10.6 ± 1.1 n.s.
MFI 910 ± 37 1577 ± 84 <0.0001 745 ± 41 1544 ± 134 <0.0001 1064 ± 63 1869 ± 139 <0.0001 1023 ± 56 1968 ± 156 <0.0001
IL-6 % positive 27.0 ± 2.4 26.4 ± 3.5 n.s. 25.7 ± 1.6 22.1 ± 3.3 n.s. 49.7 ± 3.3 37.2 ± 5.1 n.s. 59.5 ± 2.8 41.5 ± 4.7 n.s.
MFI 537 ± 73 792 ± 164 n.s. 545 ± 76 790 ± 163 n.s. 652 ± 83 827 ± 163 n.s. 722 ± 91 882 ± 170 n.s.
TNF-α % positive 18.3 ± 2.4 18.2 ± 2.1 n.s. 23.7 ± 2.4 16.2 ± 1.8 n.s. 74.6 ± 3.9 62.0 ± 4.4 n.s. 86.4 ± 1.8 78.9 ± 2.3 n.s.
MFI 2214 ± 486 1285 ± 239 0.006 2315 ± 508 1313 ± 221 0.006 5783 ± 736 2835 ± 295 <0.0001 7509 ± 1009 3881 ± 363 <0.0001
TOTAL % positive 35.4 ± 2.2 38.3 ± 2.7 n.s. 34.9 ± 1.8 29.4 ± 3.2 n.s. 75.6 ± 3.0 66.3 ± 4.3 n.s. 84.3 ± 1.8 79.6 ± 2.1 n.s.
Monocytes - WB
Cytokine Parameter measured PAM LPS 3M-002 3M-003
Adult Neonate q-value Adult Neonate q-value Adult Neonate q-value Adult Neonate q-value
IL-12/23p40 % positive 21.6 ± 2.2 6.0 ± 0.7 <0.0001 44.9 ± 2.7 5.9 ± 0.9 <0.0001 17.4 ± 3.1 7.1 ± 1.1 0.0073 76.3 ± 2.2 24.7 ± 3.4 <0.0001
MFI 1525 ± 145 1799 ± 153 n.s. 2203 ± 184 1897 ± 162 n.s. 1557 ± 136 1889 ± 170 n.s. 4603 ± 507 2303 ± 178 <0.0009
IL-6 % positive 28.2 ± 4.0 18.1 ± 3.3 n.s. 51.3 ± 4.1 22.8 ± 3.8 <0.0001 5.1 ± 0.7 9.8 ± 1.8 n.s. 45.6 ± 4.7 34.8 ± 4.5 n.s.
MFI 548 ± 61 683 ± 124 n.s. 647 ± 68 685 ± 118 n.s. 475 ± 45 697 ± 121 n.s. 675 ± 74 806 ± 129 n.s.
TNF-α % positive 25.3 ± 2.1 10.2 ± 1.4 <0.0001 65.6 ± 2.5 16.3 ± 2.4 <0.0001 20.3 ± 3.2 13.2 ± 1.8 n.s. 88.5 ± 1.4 58.1 ± 4.6 <0.0001
MFI 1896 ± 395 1865 ± 549 n.s. 3056 ± 423 1760 ± 471 <0.0009 1996 ± 354 2089 ± 436 n.s. 7622 ± 885 3355 ± 559 <0.0009
TOTAL % positive 44.7 ± 3.0 24.9 ± 3.0 <0.0001 76.8 ± 1.5 32.7 ± 3.5 <0.0001 27.2 ± 3.1 20.3 ± 2.4 n.s. 88.5 ± 1.2 65.8 ± 4.1 <0.0001
cDC - MC
Cytokine Parameter measured PAM LPS 3M-002 3M-003
Adult Neonate q-value Adult Neonate q-value Adult Neonate q-value Adult Neonate q-value
IL-12/23p40 % positive 7.2 ± 0.7 9.7 ± 1.0 n.s 3.3 ± 0.4 5.2 ± 0.6 n.s. 21.9 ± 2.9 19.2 ± 2.1 n.s. 32.0 ± 3.5 26.6 ± 2.0 n.s.
MFI 814 ± 38 1682 ± 140 <0.0001 882 ± 48 1794 ± 135 <0.0001 2114 ± 169 2808 ± 191 0.003 3599 ± 291 3929 ± 240 n.s.
IL-6 % positive 21.6 ± 2.4 14.8 ± 1.8 n.s. 21.2 ± 1.5 12.1 ± 1.3 <0.0003 17.8 ± 1.6 12.6 ± 2.2 n.s. 26.6 ± 1.6 17.4 ± 2.4 0.0036
MFI 598 ± 98 555 ± 102 n.s. 562 ± 90 511 ± 88 n.s. 557 ± 85 505 ± 101 n.s. 614 ± 92 519 ± 100 n.s.
TNF-α % positive 26.1 ± 3.3 13.9 ± 1.5 n.s. 30.7 ± 2.8 16.7 ± 2.1 0.0025 48.7 ± 3.9 46.4 ± 4.4 n.s. 63.8 ± 3.1 60.3 ± 4.1 n.s.
MFI 4487 ± 827 2975 ± 578 0.0091 4826 ± 942 3150 ± 639 0.0048 7275 ± 950 4784 ± 668 0.0005 10026 ± 1069 5734 ± 683 <0.0001
TOTAL % positive 34.1 ± 3.1 24.6 ± 2.0 n.s 37.2 ± 2.5 23.6 ± 2.3 0.0024 52.9 ± 3.6 50.1 ± 4.4 n.s. 65.8 ± 2.9 63.7 ± 3.9 n.s.
cDC - WB
Cytokine Parameter measured PAM LPS 3M-002 3M-003
Adult Neonate q-value Adult Neonate q-value Adult Neonate q-value Adult Neonate q-value
IL-12/23p40 % positive 21.4 ± 2.6 11.7 ± 1.7 0.0053 35.3 ± 2.7 12.3 ± 1.8 <0.0001 19.3 ± 2.5 16.9 ± 2.3 n.s. 67.3 ± 3.7 31.1 ± 3.8 <0.0001
MFI 1799 ± 180 2582 ± 282 n.s. 2470 ± 215 2893 ± 282 2264 ± 212 3497 ± 306 n.s. 6827 ± 852 3985 ± 335 n.s.
IL-6 % positive 21.7 ± 3.1 14.4 ± 2.4 n.s. 32.6 ± 2.7 17.5 ± 2.4 0.0003 5.4 ± 0.6 10.3 ± 1.6 n.s. 17.6 ± 2.4 15.7 ± 2.0 n.s.
MFI 456 ± 73 577 ± 112 n.s. 509 ± 72 591 ± 110 357 ± 49 621 ± 116 n.s. 412 ± 56 620 ± 112 n.s.
TNF-α % positive 26.4 ± 3.1 12.7 ± 1.8 0.0002 52.1 ± 2.9 18.0 ± 2.3 <0.0001 22.4 ± 2.6 21.4 ± 3.0 n.s. 73.8 ± 3.6 43.3 ± 4.9 <0.0001
MFI 3515 ± 617 3685 ± 832 n.s. 4425 ± 585 3639 ± 727 3280 ± 457 3853 ± 683 n.s. 6151 ± 585 4176 ± 633 n.s.
TOTAL % positive 39.3 ± 3.5 24.0 ± 2.7 0.0031 61.1 ± 2.7 30.5 ± 3.0 <0.0001 28.0 ± 2.7 28.5 ± 3.3 n.s. 76.3 ± 3.2 53.2 ± 4.7 <0.0001
pDC - MC
Cytokine Parameter measured 3M-002 3M-003 3M-013 CpG-A
Adult Neonate q-value Adult Neonate q-value Adult Neonate q-value Adult Neonate q-value
IFN-α % positive 3.6 ± 0.9 1.3 ± 0.3 n.s. 58.1 ± 2.2 13.5 ± 1.8 <0.0003 32.3 ± 2.9 7.0 ± 1.0 <0.0003 40.9 ± 3.6 9.8 ± 1.3 <0.0003
MFI 2314 ± 1926 562 ± 92 0.0072 1784 ± 237 833 ± 46 <0.0001 1509 ± 225 1089 ± 166 0.005 2978 ± 239 1661 ± 148 <0.0001
IL-6 % positive 1.6 ± 0.3 1.5 ± 0.2 n.s. 3.3 ± 0.4 2.4 ± 0.3 n.s. 3.3 ± 0.3 1.4 ± 0.2 <0.0003 2.3 ± 0.5 1.1 ± 0.2 n.s.
MFI 1537 ± 1277 219 ± 31 n.s. 330 ± 54 235 ± 23 n.s. 448 ± 170 226 ± 25 n.s. 233 ± 33 390 ± 127 n.s.
TNF-α % positive 6.7 ± 1.3 9.2 ± 1.7 n.s. 52.3 ± 2.7 26.2 ± 2.1 <0.0003 54.8 ± 3.1 22.2 ± 2.1 <0.0003 27.5 ± 2.8 9.2 ± 1.2 <0.0003
MFI 1786 ± 381 1077 ± 171 n.s. 2379 ± 454 1188 ± 197 0.0002 2622 ± 498 1058 ± 206 <0.0001 2326 ± 505 1008 ± 182 <0.0001
TOTAL % positive 10.2 ± 1.4 10.7 ± 1.6 n.s. 70.1 ± 1.7 32.2 ± 2.0 <0.0003 60.1 ± 2.8 24.7 ± 2.2 <0.0003 45.8 ± 3.7 15.5 ± 1.6 <0.0003
pDC - WB
Cytokine Parameter measured 3M-002 3M-003 3M-013 CpG-A
Adult Neonate q-value Adult Neonate q-value Adult Neonate q-value Adult Neonate q-value
IFN-α % positive 1.4 ± 0.2 1.9 ± 0.3 n.s. 50 ± 2.9 19 ± 1.3 <0.0001 15 ± 2.0 6.7 ± 0.6 <0.0001 2.6 ± 0.6 3.6 ± 0.6 n.s.
MFI 2448 ± 1959 439 ± 144 n.s. 2156 ± 301 835 ± 73 <0.0009 1359 ± 228 645 ± 69 n.s. 1372 ± 42 3057 ± 2057 n.s.
IL-6 % positive 1.4 ± 0.3 1.5 ± 0.3 n.s. 2.8 ± 0.6 3.0 ± 0.5 n.s. 2.6 ± 0.4 2.2 ± 0.4 n.s. 1.1 ± 0.2 1.3 ± 0.2 n.s.
MFI 223 ± 28 248 ± 55 n.s. 226 ± 24 195 ± 18 n.s. 210 ± 24 218 ± 27 n.s. 283 ± 75 793 ± 595 n.s.
TNF-α % positive 3.1 ± 0.5 3.0 ± 0.6 n.s. 51.1 ± 3.1 22.5 ± 2.1 <0.0001 47.2 ± 2.9 20.7 ± 2.3 <0.0001 3.3 ± 0.5 2.5 ± 0.5 n.s.
MFI 835 ± 136 977 ± 117 n.s. 1687 ± 259 1043 ± 121 n.s. 1603 ± 267 916 ± 121 n.s. 1012 ± 246 855 ± 133 n.s.
TOTAL % positive 5.1 ± 0.5 5.2 ± 0.6 n.s. 57.2 ± 2.7 31.4 ± 1.9 <0.0001 48.9 ± 2.7 24.7 ± 2.1 <0.0001 5.5 ± 0.7 6.1 ± 0.9 n.s.
a

Shown are the mean ± SD of percentage and the mean fluorescence intensity (MFI) of cells producing an individual cytokine, as well as the total percentage of cells producing any cytokine. The probability that differences are significant after correction for multiple comparisons is shown as q-values (see statistical methods for description).

n.s. = non-significant.