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(a) Schematic of analyte diffusion onto walls of well during sandwich assay. (b) Schematic of interdroplet distance as measured from droplet concentration of 5×1015 m−3. (c) Integrated brightness vs. time from HIFU pulsing of droplets with 1 µM (black circles), 1 nM (red squares), or 1 pM (green triangles) streptavidin added at time 0. Error bar = 1 SD, study was run in triplicate.
