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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Biochemistry. 2008 Aug 15;47(36):9540–9552. doi: 10.1021/bi800544d

Figure 10.

Figure 10

n-Butanol inhibits the extraction of Cdc42 from membranes by RhoGDIα. A, The emission fluorescence intensity of MANT-GMPPNP (2 µM) loaded on Cdc42 (60 nM), at 430 nm upon excitation at 355 nm, was measured in the presence of LUV composed of POPC/POPS (4:1, molar) containing 2 µM HAF. The recovery of fluorescence from quenching due to MANT–HAF FRET upon addition of 60 nM RhoGDIα was monitored as a function of time in the presence (bold) and absence of 50 mM n-butanol. Results are representative of experiments carried out at least three times. Other details are described in “Experimental Procedures.”