Figure 5.

Concentration dependent effects of n-butanol on the quenching of RhoGDIα tryptophan emission fluorescence by varying levels of halothane. Tryptophan emission fluorescence recovery at 330 nm upon excitation at 290 nm was measured as a function of n-butanol concentration in the presence of 0.1 mM (●), 0.2 mM (▪), 0.5 mM (▲) and 1 mM (▼) halothane. The solid curves represent fits of the data to Eq. 2 using least squares non-linear regression analysis. Values of EC₅₀, h and F_max are listed in Table 2. Data are representative of three independent experiments. The fits of each individual replicate experiment yielded similar parameter values and errors. Other details are described in “Experimental Procedures.”