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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Biochemistry. 2008 Aug 15;47(36):9540–9552. doi: 10.1021/bi800544d

Figure 6.

Figure 6

Identification of E193 as a site of photoincorporation of 3-azibutanol (100 µM) on RhoGDIα (2.8 nM) in the presence of AGGC (550 nM) using LC–MSMS. After photolabeling, RhoGDIα was digested with trypsin and subject to HPLC–MSMS as described in the text. Inset, a tryptic peptide bearing a single 72 Da modification and starting at F181 of RhoGDIα. The predicted charge/mass ratios of ions with an intact N-terminus (b-ions) or C-terminus (y-ions) are shown above and below the sequence, respectively, with the indicated charge. The site of photoincorporation for 3-azibutanol was inferred from the MSMS spectrum. The experimentally observed values are colored and in bold and their position indicated on the spectrum. The photolabeled residue is boxed and the statistics are given in Table 3.