Skip to main content
. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Biochemistry. 2008 Aug 15;47(36):9540–9552. doi: 10.1021/bi800544d

Figure 7.

Figure 7

Identification by LC-MSMS of E163 as a photoincorporation site for 3-azioctanol (10 µM) on RhoGDIα (2.8 nM) in the absence of AGGC. After photolabeling, RhoGDIα was digested with trypsin and subject to HPLC–MSMS as described in the text. A tryptic peptide bearing a single 128 Dalton modification and starting at A153 of RhoGDIα had the sequence shown in the inset. Statistics are given in Table 3. The site of photoincorporation of 3-azibutanol was inferred from this MSMS spectrum. In the inset, the predicted charge/mass ratios of ions with an intact N-terminus (b-ions) or C-terminus (y-ions) are shown above and below the sequence, respectively, with the indicated charge. The photolabeled residue is boxed. The experimentally observed values are colored and in bold and their position indicated on the spectrum.