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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Biochemistry. 2008 Aug 15;47(36):9540–9552. doi: 10.1021/bi800544d

Figure 8.

Figure 8

Identification by LC-MSMS of E163 as a photoincorporation site for 3-azibutanol (100 µM) on RhoGDIα (2.8 nM) in the absence of AGGC. After photolabeling, RhoGDIα was digested with trypsin and subject to HPLC–MSMS as described in the text. A tryptic peptide bearing a single 72 Dalton modification and starting at A153 of RhoGDIα had the sequence shown in the inset. Statistics are given in Table 3. The site of photoincorporation for 3-azibutanol was inferred from this MSMS spectrum. The identified b- and y-ions are indicated above the MS/MS spectrum using the same conventions as in Figure 7.