Table 1.

Chain Length Dependent Effects of n-Alkanols on Halothane Binding and RhoGDIα– Cdc42 Complex Dissociation.

	Halothane Bindinga				Cdc42–RhoGDIα Dissociationb
n	EC50, mM	h	Fmax	KIc, mM	Kapp (mM)	h
2	100 ± 58	1.4 ± 0.4	44 ± 5	42 ± 24	70 ± 21	1.2 ± 0.2
3	48 ± 4.5	1.2 ± 0.2	40 ± 9	20 ± 2	ndd	ndd
4	10 ± 3	1.1 ± 0.1	51 ± 4	4.2 ± 1.2	7.5 ± 1.3	1.5 ± 0.3
5	38 ± 19	1.1 ± 0.2	41 ± 8	16 ± 8	ndd	ndd

^aValues of EC₅₀, F_max, h for the displacement of halothane binding were obtained from fits of the dose-response curves shown in Figure 3 to Eq. 2.

^bK_app and h for the potentiation of Cdc42– RhoGDIα dissociation were obtained from dose-response curves shown in Figure 6 using Eq. 6.

^cValues of K_I were calculated using Eq. 3.

^dnot determined. See “Experimental Procedures” section for other details.