Table 4.

Sequence Coverage of RhoGDIα Following In-Gel Digestion with Trypsin.^a

001 MAEQEPTAEQ LAQIAAENEE DEHSVNYKPP AQKSIQEIQE LDKDDESLRK YKEALLGRVA VSADPNVPNV 071 VVTGLTLVCS SAPGPLELDL TGDLESFKKQ SFVLKEGVEY RIKISFRVNR EIVSGMKYIQ HTYRKGVKID 141 KTDYMVGSYG PRAEEYEFLT PVEEAPKGML ARGSYSIKSR FTDDDKTDHL SWEWNLTIKK DWKD
			m/z		Accuracyb (ppm)
Position	Sequence	Charge	Observed	Calculated
34–49	SIQEIQELDKDDESLR	2	959.4602	959.4736	14.0
34–50	SIQEIQELDKDDESLRK	2	1023.5096	1023.5211	11.2
51–58	YKEALLGR	1	949.5387	949.5465	8.2
53–58	EALLGR	1	658.3844	658.3883	5.9
59–99	VAVSADPNVPNVVVTGLTLVCSSAPGPLELDLTGDLESFKK	4	1039.5316	1039.5526	20.2
99–105	KQSFVLK	1	849.5158	849.5193	4.1
100–105	QSFVLK	1	721.4184	721.4243	8.2
106–111	EGVEYR	1	752.3508	752.3573	8.6
114–117	ISFR	1	522.3012	522.3034	4.2
118–127	VNREIVSGMK	1	1132.617	1132.6143	2.4
121–127	EIVSGMK	1	763.394	763.4019	10.3
128–134	YIQHTYR	1	980.4887	980.4948	6.2
128–135	YIQHTYRK	1	1108.5764	1108.5898	12.1
139–152	IDKTDYMVGSYGPR	2	801.3766	801.385	10.5
142–152	TDYMVGSYGPR	2	623.2784	623.2821	5.9
153–167	AEEYEFLTPVEEAPK	2	876.4141	876.4223	9.4
168–172	GMLAR	1	547.299	547.3021	5.7
173–178	GSYSIK	1	654.3412	654.3457	6.9
179–199	SRFTDDDKTDHLSWEWNLTIK	3	870.0851	870.0884	3.8
181–199	FTDDDKTDHLSWEWNLTIK	3	789.0358	789.0441	10.5
181–200	FTDDDKTDHLSWEWNLTIKK	3	831.7341	831.7424	10.0
187–199	TDHLSWEWNLTIK	3	548.2857	548.279	12.2

^aThe sequence of the RhoGDIα is shown in single letter code. The proteolytic fragments sequenced by MSMS are shown in bold. The sequence coverage by amino acid count is 79.4%;

^bAccuracy is the difference between the calculated and observed m/z expressed in ppm of the calculated value.