. 2015 Aug 25;71(Pt 9):1946–1954. doi: 10.1107/S1399004715013760

Table 3. The pK _a values for the catalytic base residues of C. bescii PL3 and B.subtilis PL1 indicate that the local chemical environment, as well as the amino-acid identity, determine the optimal pH ranges for each enzyme.

PDB code	Apo/holo	Organism	PROPKA	H++
C. bescii PL3, Lys108
3t9g	Apo	C. bescii	9.7	>12
4ew9	Holo^†	C. bescii	8.7	7.2
B. subtilis PL1, Arg279
1bn8	Apo	B. subtilis	12.0	>12
2nzm	Holo	B. subtilis	9.1	9.1

^†

4ew9 represents PL3 with reacted ligand or product.

Table 3. The pK a values for the catalytic base residues of C. bescii PL3 and B.subtilis PL1 indicate that the local chemical environment, as well as the amino-acid identity, determine the optimal pH ranges for each enzyme.