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. Author manuscript; available in PMC: 2016 Aug 13.
Published in final edited form as: Cell. 2015 Aug 13;162(4):872–884. doi: 10.1016/j.cell.2015.07.041

Figure 1. Ribosomes accumulate at stop codons and in 3′UTRs of most genes when Rli1 is depleted.

Figure 1

(A) Normalized average ribosome footprint occupancy (each gene equally weighted) from all genes aligned at their stop codons for WT, SUP4-o (genes with UAA stop codons only) and rli1-d cells. (Inset) Demagnified view of (A) with schematic depicting ribosome stalling and queuing at the stop codon. (B) Ratio of footprint densities in 3′UTRs to the respective ORFs is plotted for rli1-d cells versus WT cells, for genes with >5 rpkm in ORFs and >0.5 rpkm in 3′UTRs. Each point represents the data for 1 gene.