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. Author manuscript; available in PMC: 2016 Aug 19.
Published in final edited form as: ACS Chem Neurosci. 2015 Jun 12;6(8):1476–1485. doi: 10.1021/acschemneuro.5b00134

FIGURE 5. Pharmacological effects of CY chemical ligands in hnRNPA2B1 and M-opsin proteostasis.

FIGURE 5

(a) Immunoblot (top panel) and quantitative analyses (lower panel) of hnRNPA2B1 levels in homogenates of STAT3 luciferase reporter HeLa stable cells untreated and treated with small molecules (100 μM). Compounds 11–13 reduce strongly hnRNPA2B1 levels. Compound 14 is cytotoxic. (b) Quantitative analyses (upper graph) by immunoblot of hnRNPA2B1 levels (lower panel) in homogenates of HeLa cells untreated and treated with increasing concentrations of compound 11. (c) Immunoblot (upper panel) and quantitative analyses (lower panel) of M-opsin levels in homogenates of 661W cells untreated and treated with small molecules. Compounds 2 and 13 reduce and increase M-opsin levels, respectively. Data shown represent the mean ± s.d., n = 4 (a–c). Legend: V, vehicle (compound solvent) only; Cmp, compound (small molecule); Gapdh, Glyceraldehyde 3-phosphate dehydrogenase; hsc70, cytosolic heat shock cognate protein 70.