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. 2015 Jun 25;106(8):972–981. doi: 10.1111/cas.12700

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© 2015 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Fetal/neonatal activation of tumor-associated genes and their products. (a) Quantitative RT-PCR analyses of mRNA expression of tumor-associated genes during fetal/neonatal periods. Each value is expressed as the mean ± SEM. The number of samples in each group was 3, 5, 7, 10, 4, 4, 4, and 7 for E13.5, E16.5, P0 (immediately after birth), P1 (1 day after birth), P3 (3 days after birth), P6 (6 days after birth), 1 m (1 month old), and 5 m (5 months old), respectively. *P < 0.05, **P < 0.01, ***P < 0.001 versus control (5 m); one-way factorial anova. (b) In situ detection of insulin-like growth factor 2 (IGF2), H19 mRNA, trefoil factor 3 (TFF3), and α-fetoprotein (AFP) in developing livers. Immunohistochemistry for IGF2, TFF3, and AFP and in situ hybridization for H19 mRNA in fetal (E16.5) and neonatal (P0) livers. Scale bar = 20 μm.