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. 2015 Jun 25;106(8):972–981. doi: 10.1111/cas.12700

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© 2015 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Differential gene expression in thioacetamide (TAA)-induced liver tumors and spontaneously developed liver tumors in mice. (a) Histology of liver tissues from TAA-induced and spontaneous tumors. The ages of mice at analyses were 38–40 weeks and 13–15 months in the TAA-induced model and spontaneous model, respectively. HE staining. NT, non-tumor; T, tumor. Scale bar = 50 μm. (b) Quantitative RT-PCR analyses of mRNA expression of 15 tumor-associated genes in TAA-induced and spontaneous (Spont.) tumors. Each value is expressed as the mean ± SEM. The number of samples in each group was 7, 6, 9, 4, and 3 for the TAA control (C), TAA-induced cirrhosis (NT), TAA-induced tumors (T), control C3H mouse liver (NT), and spontaneous tumors (T) in C3H mice, respectively. *P < 0.05, **P < 0.01, ***P < 0.001 versus control; one-way factorial anova.