Figure 1.

BRCA1-associated protein 1 (BAP1) gene mutations in malignant mesothelioma (MM). (a) Schematic diagram of BAP1 mutations in MM cell lines. (b) Schematic diagram of an insertion mutation due to G to T change at the acceptor site of exon 8 in Y-MESO-61. (c) Genomic PCR analysis of exons of BAP1 detected homozygous deletion (HD) in Y-MESO-25 cells (arrowhead). (d) Western blot analysis of BAP1. Expression of β-actin was used as the control. (e) Summary of five gene mutation statuses in MM cell lines. Red boxes indicate inactivating mutation or HD. The mutation statuses of neurofibromatosis type 2 (NF2), large tumor suppressor homolog 2 (LATS2), Salvador homolog 1 (SAV1), and cyclin-dependent kinase inhibitor 2A (CDKN2A) were previously reported.¹¹ NLS, nuclear localization signal; UCH, ubiquitin COOH-terminal hydrolase.