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. 2015 Jul 14;106(8):1023–1032. doi: 10.1111/cas.12712

Figure 1.

Figure 1

Induction of heme oxygenase-1 (HO-1) expression mediated doxorubicin (DOX) resistance in MDA-MB-231 cells. (a) MDA-MB-231 and MDA-MB-453 cells were left untreated or treated with DOX (0.2 μM) for 48 h and then cell death incidence was determined by flow cytrometric assay after propidium iodide (PI) staining of the nuclei. (b) MDA-MB-231 and MDA-MB-453 cells were treated with DOX (0.2 μM) for the indicated time periods and then the induction of HO-1 expression was detected by western-blot assay. (c) MDA-MB-231 cells were transfected with HO-1 siRNA or control siRNA and then treated with DOX (0.2 μM) for the indicated time periods 36 h after transfection. The efficiency of HO-1 siRNA was determined by western-blot assay. To avoid the off-target effect, two different siRNA against HO-1 were purchased and tested. Data shown here were obtained from the most effective one. (d) MDA-MB-231 cells were transfected and treated with DOX as described in (c) and then cell death incidence was detected 48 h after DOX treatment.