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. 2015 Jul 14;106(8):1023–1032. doi: 10.1111/cas.12712

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© 2015 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Doxorubicin (DOX)-induced autophagy was dependent on heme oxygenase-1 (HO-1). (a) MDA-MB-231 cells were transfected and treated as described in Figure1(c) and then the expression levels of Beclin-1 and LC3BI/II were detected. The image shown in this figure and Figure1(c) were developed from the same membrane. (b,c) MDA-MB-231 cells were transfected and treated as described in Figure1(c) and then autophagy was detected either under confocal microscopy (b) or by flow cytometric analysis (c) after staining the cells with Cyto-ID Green Autophagy Detection Reagent.