Figure 1.

Upregulation of miR-200 family members by the p53 family in human cancer cell lines. Cells were infected with recombinant adenoviruses encoding human p53 family at an MOI of 25 (H1299), 12.5 (Saos2) or 50 (U2OS) and harvested 24 h after infection. The relative gene expression levels were quantified using the ΔΔC_t method via real-time RT-PCR, and the results were normalized to the expression of the housekeeping gene RNU6B. The data are shown as the mean ± standard error of three independent experiments and were normalized to their respective controls as 1.