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. 2015 Jul 14;106(8):1033–1040. doi: 10.1111/cas.12713

Figure 4.

Figure 4

The CRKL 3′-UTR is regulated by the p53 family in H1299 and Saos2 cells. Cells were transiently transfected with the pMIR-REPORT vector containing CRKL 3′-UTR (pMIR-CRKL-3′-UTR) or a mutant (pMIR-CRKL-3′-UTR-mut) along with transfection control plasmids expressing Renilla luciferase. The cells were co-transfected with a control pcDNA3.1 vector or a vector that expressed the p53 family member 48 h prior to performing the luciferase assay. Luciferase activity was measured via the dual-luciferase reporter assay system. All of the experiments were performed in quadruplicate, and the mean and standard deviation are indicated by the bars.