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. 2015 Aug 31;10(8):e0136707. doi: 10.1371/journal.pone.0136707

Fig 1. Two- dimensional electrophoretic (2-DE) profile of the soluble fractions of parental and sodA mutants strains of B. burgdorferi.

Fig 1

Soluble proteins of B. burgdorferi parental (wt) and sodA mutant (mt) strains before (Control) and after exposure to 20 mM MV (Treated) were separated from membrane proteins by ultracentrifugation and resolved using IPG strips (pH 5–8) in the first dimension and by SDS-12% PAGE gels in the second dimension. Following 2-DE, the total proteins were stained with SYPRO Ruby (Molecular Probes) with basic and acid ends indicated for each gel. Numbers with lines represent the spots that correlate with oxidized proteins detected by immunoblot in Fig 2. Spots were excised and analyzed by MALDI-TOF MS. Results are outlined in Table 1 with spot number indicated within parenthesis. Molecular mass markers (MW) are represented on the left side of each gel in kDa. Representative gels are presented in this figure.