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. 2015 Aug 31;10(8):e0136707. doi: 10.1371/journal.pone.0136707

Fig 2. Immunoblot analysis of oxidized soluble proteins of parental and sodA mutant strains of B. burgdorferi.

Fig 2

Soluble proteins from the parental strain (wt) and sodA mutant (mt) were obtained before (Control) and after treatment with 20 mM MV (Treated) and subjected to two-dimensional gel electrophoresis after derivatization with dinitrophenylhydrazine (DNPH) as described in the material and methods. The proteins were transferred to PVDF membranes and probed with anti-DNP antiserum and blots developed using Enhanced Chemiluminescence Plus Detection reagents. Lines and numbers represent the protein spots oxidized in the sodA mutant compared with the parental strain. Spots were excised from the SYPRO stained gels as shown in Fig 1 and analyzed by MALDI-TOF MS. Molecular mass markers (MW) are represented on the left side of each gel in kilodaltons, OxyBlot Protein standard was used in this assay (Chemicon International, Inc.). Representative blots are presented in this figure.