Table 1. Proteins with increased levels of oxidation in the sodA mutant identified by mass spectrometric analysis.
| Peptides identified | Coverage (%) | |||||||
|---|---|---|---|---|---|---|---|---|
| Protein ID* | pI | Size (KDa) | Exp 1 | Exp 2 | Exp 1 | Exp 2 | Gene Locus | Function |
| • Glyceraldehyde 6-phosphate dehydrogenase (GAPDH) (4) | 8.2207 | 36.25 | 4 | 15 | 34 | 45 | bb0057 | Glycolysis |
| • Pyrophophate phospho fructo kinase (2) | 6.5319 | 62.48 | 9 | 11 | 14 | 20 | bb0020 | Glycolysis |
| • Pyruvate kinase (Pky) (3) | 7.23 | 53.03 | 22 | 5 | 32 | 10 | bb0348 | Glycolysis |
| • Chaperon hp90 (HtpG) (1) | 5.3925 | 75.43 | 14 | 15 | 22 | 25 | bb0560 | Protein folding |
| • Outer Membrane protein A(5) | 9.35 | 29.35 | 7 | ND | 27 | ND | Bba015 | Cell envelop, Lipoprotein |
*: The numbers of the spots in the gel shown in Fig 2 are indicated within parentheses.
ND: Not determined