Table 1. Bacterial strains and sera used for this study.
| Strain or serum | Description * | Reference or source |
|---|---|---|
| Strains | ||
| E. faecium E155 | ARE, VRE strain isolated from a patient in the USA (Chicago), CC17 | [17] |
| E. faecium E1162 | ARE strain isolated from blood in the Netherlands, CC17 | [18] |
| E. faecalis 12030 | isolated from a patient in the USA (Cleveland) | [19] |
| E. faecalis type 2 | isolated from a patient in Japan (Sapporo) | [20] |
| E. faecalis type 5 | isolated from a patient in Japan (Kobe) | [20] |
| E. coli M15pRep4 | M15 harboring pRep4 plasmid | (INVITROGEN) |
| E. coli M15/pQE30AdcA fm | M15 harboring pRep4 and pQE30AdcAfm plasmids | This study |
| E. coli M15/pQE30PsaA fm | M15 harboring pRep4 and pQE30PsaAfm plasmids | This study |
| Sera | ||
| NRS | Pre-immune sera from rabbit | This study |
| Anti-SagA | Rabbit serum raised against the recombinant SagA | [21] |
| NRS-AdcA fm | Pre-immune sera from rabbit immunized with AdcAfm collected at day 0 | This study |
| Test-AdcA fm | Rabbit serum raised against the recombinant AdcAfm collected at day 45 | This study |
| Anti-AdcA fm | Rabbit serum raised against the recombinant AdcAfm collected at day 63 | This study |
| NRS-PsaA fm | Pre-immune sera from rabbit immunized with PsaAfm collected at day 0 | This study |
| Test-PsaA fm | Rabbit serum raised against the recombinant PsaAfm collected at day 45 | This study |
| Anti-PsaA fm | Rabbit serum raised against the recombinant PsaAfm collected at day 63 | This study |
* AdcAfm, zinc ABC transporter substrate-binding lipoprotein from E. faecium; ARE, ampicillin resistant enterococci; CC17, clonal linage complex 17; PsaAfm, manganese ABC transporter substrate-binding lipoprotein from E. faecium; SagA; major secreted antigen; VRE, vancomycin resistant enterococci.