Figure 1.

TC3.6 increases the levels of cAMP in microglia culture and modulates the anti-inflammatory response. (A) Chemical compounds used. (B) Intracellular levels of cAMP measured in rat primary microglial cultures in the presence of TC3.6 and BRL50481 (10 or 30 μM). Values represent the means ± SD from three different experiments. *P < 0.05, **P < 0.01 versus control. (C) Nitrite production in rat primary microglial cultures treated with LPS (10 μg·mL⁻¹) in the absence or presence of TC3.6 and BRL50481 (30 μM). Some of the cultures were pretreated with the PKA inhibitor H-89 or the cAMP antagonist Rp-cAMP. Values represent the means ± SD of six replications in three different experiments. ***P < 0.001 versus LPS-treated cells.