Figure 3.

Mutation of the PKCε phosphorylation sites impaired α₄β₂ nAChR recovery from deep desensitization. (A–E) Recovery from desensitization induced by ACh (100 μM, 60 s) in native and mutant α₄β₂ nACh receptors containing PKCε phosphorylation site point mutations. (F) Mean current density (pA·pF⁻¹) for the native and mutant receptors. Native α₄β₂ n = 14, S465A n = 12, S467A n = 9, T545A n = 9, S550A n = 13, T551A n = 9 cells. (G) Activation and (H) deactivation time for native and mutant receptors. ^*P < 0.05, ^**P < 0.01 compared with the native receptor. Native α₄β₂ n = 11, S465A n = 8, S467A n = 9, T545A n = 8, S550A n = 9, T551A n = 9 cells.