. 2015 Sep 1;10(9):e0136298. doi: 10.1371/journal.pone.0136298

Table 1. Summary of immune cell phenotypes of mice splenocytes detected by flow cytometry.

Results are expressed as percentage (mean ± SD) of total splenocytes from 331 G4 mice. MHC II: major histocompatibility complex class II, FoxP3: forkhead box protein P3, IFNγ: interferon-γ, IL4: interleukin-4, IL17: interleukin-17, PDCA1: plasmacytoid dendritic cell antigen-1. Cell types were defined as follows: B cells–CD19⁺, T cells–CD3⁺, double-positive (DP) T cells–CD4⁺/CD8⁺, cytotoxic T cells–CD8⁺, T helper cells–CD4⁺, Th1 cells–CD4⁺/IFNγ⁺, Th2 cells—CD4⁺/IL4⁺, Th17 cells—CD4⁺/IL17⁺, Tregs–FoxP3⁺, conventional dendritic cells (cDCs)–CD11c⁺, Plasmacytoid dendritic cells (PD)–PDAC1⁺.

Cell Type			Marker	Mean ± SD
B cells			CD19⁺/CD69^-	36.81 ± 11.13
			CD19⁺/CD69⁺	0.29 ± 0.21
			CD19⁺/CD86^-	38.65 ± 9.78
			CD19⁺/CD86⁺	0.41 ± 0.36
			CD19⁺/ MHC II^-	5.15 ± 5.7
			CD19⁺/ MHC II⁺	32.51 ± 11.6
T cells			CD3⁺/CD8⁺	8.3 ± 4.06
			CD8⁺/CD44^low	4.2 ± 3.12
			CD8⁺/CD44^high	4.6 ± 3.47
	cytotoxic		CD8⁺/CD62L^-	2.5 ± 2.38
	T cells		CD8⁺/CD62L⁺	6.07 ± 3.81
			CD8⁺/CD69^-	8 ± 4.59
			CD8⁺/CD69⁺	0.38 ± 0.37
			CD8⁺/FoxP3^-	8.36 ± 4.56
	DP T cells		CD4⁺/CD8⁺	0.57 ± 0.38
		Th 1	CD4⁺/IFNγ⁺	0.48 ± 0.6
		Th 2	CD4⁺/IL4⁺	0.21 ± 0.21
		Th 17	CD4⁺/IL17⁺	0.29 ± 0.27
			CD3⁺/CD4⁺	15.5 ± 5.59
			CD4⁺/CD8^-	15.66 ± 5.6
	T helper		CD4⁺/CD69^-	16.4 ± 5.33
	cells		CD4⁺/CD69⁺	0.33 ± 0.68
		others	CD4⁺/CD44^low	4.54 ± 3.44
			CD4⁺/CD44^high	12.63 ± 4.34
			CD4⁺/CD62L^-	9.39 ± 3.48
			CD4⁺/CD62L⁺	7.54 ± 4.65
			CD4⁺/FoxP3^-	17.64 ± 6.93
			CD4⁺/IFNγ^-	17.58 ± 6.27
	Tregs		CD4⁺/FoxP3⁺	0.41 ± 0.38
			CD8⁺/FoxP3⁺	0.14 ± 0.14
			CD3⁺/CD4^-	9.81 ± 4.13
	others		CD3⁺/CD8^-	17.01 ± 5.75
			CD3⁺/B220^-	22.91 ± 8.6
			CD3⁺/B220⁺	2.32 ± 1.01
DCs			PDCA1⁺/CD86^-	3.51 ± 3.52
	PD cells		PDCA1⁺/CD86⁺	0.46 ± 0.54
			PDCA1⁺/CD11c^-	2.33 ± 2.4
			PDCA1⁺/CD11c⁺	0.76 ± 0.46
			CD11c⁺/CD86^-	2.43 ± 1.21
	cDCs		CD11c⁺/CD86⁺	0.32 ± 0.32
			CD11c⁺/PDCA1^-	1.95 ± 1.01