Fig 1. Effect of inhibition of ADAM10 on constitutive (A) and insulin-induced shedding of RAGE (B) in THP-1 macrophages.

Cell surface receptors were biotinylated and cell-conditioned media was immunoprecipitated with anti-biotin agarose, electrophoresed and immunobloted with anti-RAGE antibody or streptavidin-HRP (STP-HRP) antibody. Biotinylated RAGE in cell-conditioned media (cRAGE) was significantly reduced by addition of GM6001 (*p<0.05) and GI254023X (**p<0.01) vs control cells (A). Cells were incubated with insulin (10mIU/ml) for 24 h in the presence or absence of inhibitors and biotinylated RAGE in cell-conditioned media was quantified. Data represent the mean ± SEM. ^#p< 0.01 vs insulin-treated cells (B).