Table 4.
Nonischemic right ventricles (n = 6) vs. ischemic right ventricles (n = 6) proteomic results
| Cluster, protein | Gene | MW (kDa) | Unique peptides |
Spectral counts |
Peptide coverage, % |
AV ratio: non-ISCH RV/ISCH RV |
P, t test |
|---|---|---|---|---|---|---|---|
| Cluster 1 | −1.71 | 0.059 | |||||
| Alpha-1-antitrypsin (SERPINA1) | A1AT | 47 | 13 | 18 | 27 | ||
| Cluster 2 | −1.67 | 0.045 | |||||
| Alpha-1-antitrypsin (SERPINA1) | A1AT | 47 | 18 | 26 | 31 | ||
| Antithrombin-III (SERPINC1) | ANT3 | 53 | 8 | 10 | 13 | ||
| Cluster 3a | 1.25 | 0.054 | |||||
| WDR44b | B7Z964 | 37 | 23 | 34 | 49 | ||
| Desmin | DES | 54 | 8 | 9 | 19 | ||
| Cluster 4 | 1.61 | 0.026 | |||||
| Annexin A5 | ANXA | 36 | 10 | 13 | 27 | ||
| Mu-crystallin homolog | CRYM | 34 | 7 | 9 | 20 | ||
| Mimecan (osteoglycin) | OGN | 34 | 5 | 6 | 12 | ||
| Cluster 5 | 1.48 | 0.047 | |||||
| Annexin A5 | ANXA5 | 36 | 10 | 13 | 27 | ||
| Mu-crystallin homolog | CRYM | 34 | 7 | 9 | 20 | ||
| Mimecan (osteoglycin) | OGN | 34 | 5 | 6 | 12 |
Note
Unique peptides: no. of peptides unique to the parent protein in question identified by tandem mass spectrometry; spectral counts: no. of spectral counts identified by tandem mass spectrometry; peptide covered: percentage of the entire parent peptide identified by the digested peptide fragments identified by tandem mass spectrometry mapped back to the entire parent peptide. AV: average abundance; ISCH: ischemic; MW: molecular weight; RV: right ventricle.
a
Also weaker evidence for heme oxygenase 2 (HMOX2), lactate dehydrogenase B (LDHB), and pyruvate dehydrogenase β (PDHB).
b
Highly similar to sarcolemma-associated protein (SLMAP).