Table 5.
RNA sequencing read count results for selected proteins
| Read counts, mean ± SD | P, t test | |||||
|---|---|---|---|---|---|---|
| Gene | DON RV | N-ISCH RV | ISCH RV | Log fold difference N-ISCH RV vs. ISCH RV |
N-ISCH RV vs. ISCH RV |
DON RV vs. all RV |
| CRYMa | 944 ± 567 | 3,653 ± 1,549 | 3,226 ± 1,376 | 1.5 | 0.50 | 0.0000080 |
| WDR44 | 292 ± 102 | 256 ± 64 | 232 ± 82 | 1.2 | 0.45 | 0.37 |
| DES | 327,255 ± 142,355 | 311,880 ± 82,070 | 355,684 ± 132,284 | 1.2 | 0.36 | 0.93 |
| ANXA5 | 3,195 ± 342 | 2,216 ± 692 | 1,907 ± 442 | 1.5 | 0.23 | 0.00017 |
| OGNa | 161 ± 30 | 448 ± 215 | 316 ± 176 | 1.5 | 0.13 | 0.000059 |
| SERPINA1 | 26 ± 16 | 29 ± 13 | 36 ± 14 | −1.7 | 0.25 | 0.45 |
| SLC25A6 | 2,320 ± 430 | 2,747 ± 474 | 2,877 ± 889 | −1.7 | 0.67 | 0.07 |
| MYH7 | 393,338 ± 179,483 | 596,572 ± 122,839 | 679,245 ± 211,888 | … | 0.28 | 0.03 |
| MYL3 | 102,061 ± 19,558 | 96,138 ± 23,483 | 99,311 ± 20,123 | … | 0.74 | 0.67 |
| TNNI3 | 149,014 ± 73,644 | 273,192 ± 48,428 | 325,525 ± 172,839 | … | 0.35 | 0.0052 |
| TPM1 | 165,469 ± 65,292 | 210,182 ± 36,562 | 212,764 ± 63,368 | … | 0.91 | 0.20 |
| ACTN2 | 46,455 ± 14,611 | 57,939 ± 14,723 | 53,922 ± 12,533 | … | 0.50 | 0.24 |
Note
Read counts: unadjusted read counts by RNA sequencing; DON RV: unused donor right ventricle; ISCH RV: ischemic right ventricle; N-ISCH RV: nonischemic right ventricle; T test, comparison of RNA sequencing read counts by a 2-way Student’s t test with the assumption of unequal variance and without adjustment for multiple comparisons.
a
CRYM and OGN were differentially expressed according to the DESeq method (adjusted P value < 0.05).