Severity of metastasis to the lung, draining and control LN at necropsy. A. Severity of metastasis to the lung of the indicated groups of mice. B. Severity of metastasis to the draining inguinal LN of the indicated groups of mice. C. Severity of metastasis to the draining axillary LN of the indicated groups of mice. D. Severity of metastasis to the control inguinal LN of the indicated groups of mice. E. Severity of metastasis to the control axillary LN of the indicated groups of mice. One way ANOVA with Tukey's multiple comparison test was used to determine the differences among the experimental groups. Each group contained 6-10 mice. a, different from water-drinking tumor-bearing mice immunized with cell lysate and stimulated with αGalCer, p<0.05. b, different from water-drinking tumor-bearing mice immunized with cell lysate and stimulated with αGalCer, p<0.05. c, different from water-drinking tumor-bearing mice (p<0.05), alcohol-consuming tumor-bearing (p<0.01), water-drinking tumor-bearing mice immunized with cell lysate alone (p<0.001), alcohol-consuming tumor-bearing mice immunized with cell lysate alone (p<0.001) and alcohol-consuming tumor-bearing mice immunized with cell lysate and stimulated with αGalCer (p<0.001).